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The resulting mixture was transferred to the pre-activated ultrafiltration membrane spin column, followed by washing with PBS (3 times), 1 TEAB (2 times), and finally reconstituted with TEAB

This trade-off is consistent with in human evidence that sustained energetic stress can lead to progressive myelin loss even in the absence of overt axonal pathology (Ramos-Cabrer et al., 2025), suggesting that metabolic compensation may preserve short-term function at the expense of long-term myelin integrity

Serum lipoproteins during treatment with antihypertensive drugs

For step-by-step mixing, see our peptide reconstitution guide and our notes on how to store peptides

Patti, G

(PubMed) Hager K, Marahrens A, Kenklies M, Riederer P, Munch G